Journal: Viruses

Article Title: New Adenovirus Groups in Western Palaearctic Bats

doi: 10.3390/v10080443

Figure Lengend Snippet: Bat species studied, AdV positive results, year of capture and GenBank accession numbers. 1 Abb., Bat species abbreviations, 2 OPS, Oropharyngeal swabs, 3 SS, Stool samples, 4 Capture Year, 5 GenBank Accession number for hexon sequences, 6 GenBank Accession number for DNA polymerase sequences. N/A: Not available.

Article Snippet: Five μL of nucleic acids extracted were added to 45 μL of reaction mixture containing 60 mM Tris-HCl (pH 8.5), 15 mM (NH 4 ) 2 SO 4 , 0.4 mM each of dNTPs (GE Healthcare, Buckinghamshire, UK), 60 pmol of each primer and 2.5 U AmpliTaq DNA Polymerase (Applied Biosystems, Branchburg, NJ, USA).

Techniques:

Journal: Viruses

Article Title: New Adenovirus Groups in Western Palaearctic Bats

doi: 10.3390/v10080443

Figure Lengend Snippet: Phylogenetic tree based on the analysis of the DNA-dependent DNA polymerase partial gene. Trees were estimated with MEGA 5.2 software by using the neighbour-joining method on Kimura 2 parameters model. A bootstrap test was replicated for 5000 times. Numbers represent percentage bootstrap support. GenBank accession numbers for the sequences included in the tree are as follows: BtAdV-3 (strain TJM, GU226970, Bat mastadenovirus A ), BtAdV-2 (strain PPV1, JN252129, Bat mastadenovirus B ), human AdVs: type 1 (AF534906), type 2 (J01917), type 3 (DQ086466), type 4 (AY594254), type 5 (AY339865), type 7 (AY594256), type 6 (HQ413315), type 9 (AJ854486), type 12 (X73487), type 17 (AF108105), type 19 (JQ326209), type 26 (EF153474), type 48 (EF153473), type 53 (AB605245), simian AdVs: type 1 (AY771780), type 4 (KP853121), bovine AdVs: type 2 (AF252854), type 3 (AF061654), type 1 (AC_000191), porcine AdVs: type 3 (AB026117), type 5 (AF289262), canine AdVs: type 1 (KX545420), type 2 (U77082), bat mastadenovirus: BtAdV-7 (strain WIV12, KT698856, Bat mastadenovirus D ), BtAdV-8 (strainWIV13, KT698852, Bat mastadenovirus E ), BtAdV-9 (strain WIV17, KX961095, Bat mastadenovirus F ), Rousettus leschenaultii WIV18 (NC_035072), BtAdV-4 (strain WIV9, KT698853, Bat mastadenovirus C ), Rhinolophus sinicus WIV10 (NC_029899), R. sinicus WIV11 (NC_029902), BtAdV-11 (strain 250-A, KX871230, Bat mastadenovirus G ), Plecotus austriacus Kupa (JN167523), Rhinolophus ferrumequinum Teresztenye (JN167522), Myotis spp 1419 (GU226962), R. leschenaultii 1050597 (HQ529709), Nyctalus noctula 2 119/08 (KM043096), Myotis emarginatus BS18 (KM043084), Myotis myotis BS16 (KM043106), Myotis blythii GTHU38 (KM043086), Nyctalus leisleri 239/08 (KM043102), Pipistrellus pygmaeus GTHU64 (KM043090), P. pygmaeus 176/09 (KM043091).

Article Snippet: Five μL of nucleic acids extracted were added to 45 μL of reaction mixture containing 60 mM Tris-HCl (pH 8.5), 15 mM (NH 4 ) 2 SO 4 , 0.4 mM each of dNTPs (GE Healthcare, Buckinghamshire, UK), 60 pmol of each primer and 2.5 U AmpliTaq DNA Polymerase (Applied Biosystems, Branchburg, NJ, USA).

Techniques: Software

Journal: Viruses

Article Title: New Adenovirus Groups in Western Palaearctic Bats

doi: 10.3390/v10080443

Figure Lengend Snippet: Spanish bat mastadenoviruses classified by the amino acid distance matrix analysis based on partial DNA-dependent DNA polymerase. 1 Values more than 15% are potentially new species following the demarcation criteria. Abb: P: Pipistrellus . N: Nyctalus . H: Hypsugo .

Article Snippet: Five μL of nucleic acids extracted were added to 45 μL of reaction mixture containing 60 mM Tris-HCl (pH 8.5), 15 mM (NH 4 ) 2 SO 4 , 0.4 mM each of dNTPs (GE Healthcare, Buckinghamshire, UK), 60 pmol of each primer and 2.5 U AmpliTaq DNA Polymerase (Applied Biosystems, Branchburg, NJ, USA).

Techniques: Virus

Journal: Cell reports

Article Title: Passenger Mutations Confound Phenotypes of SARM1-Deficient Mice

doi: 10.1016/j.celrep.2020.03.062

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Off- target CRISPR cleavage was accessed by PCR amplification using the primers listed in Table S5 and cycling conditions 95° 30 s, 60° 30 s, 72° 1 min and the Surveyor Mutation Detection Kit (IDT) on a pup from a cross of the Sarm1 AGS3 founder mouse to WT.

Techniques: Recombinant, Purification, Filtration, Mutagenesis, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Sample Prep, Infection, Expressing, Sequencing, Plasmid Preparation, Software